5XC6

Dengue Virus 4 NS3 Helicase in complex with SSRNA SLA12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-04-23
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54179
Spacegroup name	C 1 2 1
Unit cell lengths	132.899, 105.406, 72.694
Unit cell angles	90.00, 116.36, 90.00

Refinement procedure

Resolution	19.859 - 2.900
R-factor	0.2042
Rwork	0.201
R-free	0.25620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jlu
RMSD bond length	0.009
RMSD bond angle	1.225
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	19.860
High resolution limit [Å]	2.900
Number of reflections	19928
<I/σ(I)>	8
Completeness [%]	99.7
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	286	0.1 M MES, 15% polyethylene glycol 8000