5XC6
Dengue Virus 4 NS3 Helicase in complex with SSRNA SLA12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-04-23 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54179 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 132.899, 105.406, 72.694 |
Unit cell angles | 90.00, 116.36, 90.00 |
Refinement procedure
Resolution | 19.859 - 2.900 |
R-factor | 0.2042 |
Rwork | 0.201 |
R-free | 0.25620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jlu |
RMSD bond length | 0.009 |
RMSD bond angle | 1.225 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.860 |
High resolution limit [Å] | 2.900 |
Number of reflections | 19928 |
<I/σ(I)> | 8 |
Completeness [%] | 99.7 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 286 | 0.1 M MES, 15% polyethylene glycol 8000 |