5XA1
Crystal structure of inositol 1,4,5-trisphosphate receptor cytosolic domain with inositol 1,4,5-trisphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-02 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.00 |
Spacegroup name | P 42 |
Unit cell lengths | 126.803, 126.803, 367.784 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.500 - 6.204 |
R-factor | 0.361 |
Rwork | 0.360 |
R-free | 0.38230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5X9Z chain A and 3UJ0 chain A |
RMSD bond length | 0.019 |
RMSD bond angle | 1.968 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.500 | 6.427 |
High resolution limit [Å] | 6.200 | 6.205 |
Rmerge | 0.195 | |
Number of reflections | 13069 | 1269 |
<I/σ(I)> | 8.21 | 2.18 |
Completeness [%] | 99.6 | 98 |
Redundancy | 10.6 | 8 |
CC(1/2) | 0.971 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | lithium sulfate |