5X8R
Structure of the 30S small subunit of chloroplast ribosome from spinach
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.7 |
| Number of Particle | 187946 |
| Software | EMAN2,RELION,CTFFIND3 |
| CTF correction | PHASE FLIPPING ONLY |
