5X87
Crystal structure of a bacterial Bestrophin homolog from Klebsiella pneumoniae with a mutation L177T
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-02-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 114.485, 160.573, 162.657 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.730 - 3.140 |
R-factor | 0.2002 |
Rwork | 0.199 |
R-free | 0.23660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wd8 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.462 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.730 | 3.240 |
High resolution limit [Å] | 3.140 | 3.140 |
Rmerge | 0.061 | 2.206 |
Rpim | 0.061 | 0.859 |
Number of reflections | 101018 | 5204 |
<I/σ(I)> | 11.91 | 1.15 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 7.4 | 7.6 |
CC(1/2) | 0.998 | 0.429 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.05 M zinc acetate, 6% v/v ethylene glycol, 0.1 M sodium cacodylate, pH 6.0, 6.6 % w/v PEG 8000 |