5WZZ
The SIAH E3 ubiquitin ligases promote Wnt/ beta-catenin signaling through mediating Wnt-induced Axin degradation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-28 |
| Detector | DECTRIS PILATUS3 R CdTe 300K |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.502, 86.450, 117.454 |
| Unit cell angles | 90.00, 98.43, 90.00 |
Refinement procedure
| Resolution | 38.728 - 2.103 |
| R-factor | 0.1857 |
| Rwork | 0.184 |
| R-free | 0.20840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ca1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.371 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 92372 |
| <I/σ(I)> | 30 |
| Completeness [%] | 99.6 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 25% (w/v) PEG3350, 200 mM MgCl2, 100 mM Tris-Hcl |
