5WPL
KRas G12V, bound to GppNHp and miniprotein 225-11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.861, 234.507, 48.143 |
Unit cell angles | 90.00, 90.13, 90.00 |
Refinement procedure
Resolution | 48.140 - 2.150 |
R-factor | 0.2075 |
Rwork | 0.205 |
R-free | 0.25830 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.673 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.150 |
Number of reflections | 47914 |
<I/σ(I)> | 12.9 |
Completeness [%] | 98.1 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M ammonium sulfate and 20-25% PEG3,350 |