5WIS
Crystal structure of the Thermus thermophilus 70S ribosome in complex with methymycin and bound to mRNA and A-, P- and E-site tRNAs at 2.7A resolution
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-26 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.620, 448.780, 622.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 255.767 - 2.703 |
R-factor | 0.2242 |
Rwork | 0.222 |
R-free | 0.26640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4y4p |
RMSD bond length | 0.004 |
RMSD bond angle | 0.822 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 255.767 | 255.767 | 2.770 |
High resolution limit [Å] | 2.700 | 12.090 | 2.700 |
Rmerge | 0.142 | 0.019 | 1.185 |
Rmeas | 0.168 | 0.022 | 1.406 |
Number of reflections | 1561494 | 17891 | 110459 |
<I/σ(I)> | 8.22 | 37.04 | 1.06 |
Completeness [%] | 98.9 | 95.7 | 95 |
Redundancy | 3.408 | 3.27 | 3.388 |
CC(1/2) | 0.993 | 0.999 | 0.218 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |