5WIS

Crystal structure of the Thermus thermophilus 70S ribosome in complex with methymycin and bound to mRNA and A-, P- and E-site tRNAs at 2.7A resolution

This is a large structure.

Summary for 5WIS

Descriptor23S ribosomal RNA, 5S Ribosomal RNA, 50S ribosomal protein L2, ... (61 entities in total)
Functional Keywordsmacrolide, antibiotic, inhibitor, inhibition of translation, peptidyl transferase center, ribosome
Biological sourceEscherichia coli
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Total number of polymer chains112
Total molecular weight4572634.3
Authors
Almutairi, M.M.,Svetlov, M.S.,Hansen, D.A.,Khabibullina, N.F.,Klepacki, D.,Kang, H.Y.,Sherman, D.H.,Vazquez-Laslop, N.,Polikanov, Y.S.,Mankin, A.S. (deposition date: 2017-07-20, release date: 2018-02-14, Last modification date: 2018-03-21)
Primary citation
Almutairi, M.M.,Svetlov, M.S.,Hansen, D.A.,Khabibullina, N.F.,Klepacki, D.,Kang, H.Y.,Sherman, D.H.,Vazquez-Laslop, N.,Polikanov, Y.S.,Mankin, A.S.
Co-produced natural ketolides methymycin and pikromycin inhibit bacterial growth by preventing synthesis of a limited number of proteins.
Nucleic Acids Res., 45:9573-9582, 2017
PubMed: 28934499 (PDB entries with the same primary citation)
DOI: 10.1093/nar/gkx673
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.703 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.26680.8%2.5%7.3%0.54MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5wis
no rotation
Molmil generated image of 5wis
rotated about x axis by 90°
Molmil generated image of 5wis
rotated about y axis by 90°
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