5WHE

KRas G12V/D38P, bound to GppNHp and miniprotein 225-11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97911
Spacegroup name	P 1 21 1
Unit cell lengths	40.549, 232.525, 48.213
Unit cell angles	90.00, 90.16, 90.00

Refinement procedure

Resolution	19.540 - 1.910
R-factor	0.1866
Rwork	0.184
R-free	0.22730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gft
RMSD bond length	0.005
RMSD bond angle	0.872
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.540	1.940
High resolution limit [Å]	1.910	1.910
Rmerge	0.083
Number of reflections	65457
<I/σ(I)>	13.4
Completeness [%]	95.2
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.25 M CALCIUM CHLORIDE, 21% PEG 3350,