5WHD
Crystal structure of KRas G12V/D38P, bound to GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.239, 80.912, 108.041 |
Unit cell angles | 90.00, 90.62, 90.00 |
Refinement procedure
Resolution | 64.762 - 1.641 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.22460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gft |
RMSD bond length | 0.005 |
RMSD bond angle | 0.916 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.762 | 1.670 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmerge | 0.067 | |
Number of reflections | 74937 | |
<I/σ(I)> | 11.5 | |
Completeness [%] | 95.8 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M SODIUM FLUORIDE, 23% PEG 3350, |