5WG5

Human GRK2 in complex with Gbetagamma subunits and CCG224061

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.1271
Spacegroup name	C 2 2 21
Unit cell lengths	61.022, 240.130, 211.611
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.980 - 3.100
R-factor	0.1898
Rwork	0.188
R-free	0.23230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pnk
RMSD bond length	0.002
RMSD bond angle	0.452
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	3.100
Number of reflections	28709
<I/σ(I)>	20.8
Completeness [%]	98.0
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	50 mM MES pH 6, 8-15% PEG3350, 0.8-1.2 M NaCl