5WG3
Human GRK2 in complex with Gbetagamma subunits and CCG258748
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 61.267, 241.569, 214.813 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.071 - 2.896 |
R-factor | 0.2009 |
Rwork | 0.198 |
R-free | 0.24830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pnk |
RMSD bond length | 0.003 |
RMSD bond angle | 0.556 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.896 | 2.896 |
Rmerge | 0.132 | 0.855 |
Number of reflections | 35941 | 3411 |
<I/σ(I)> | 9.35 | 1.3 |
Completeness [%] | 99.0 | 97 |
Redundancy | 4.7 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 50 mM MES pH 6, 1.1 M NaCl, 8% PEG3350 |