5W8S
Lipid A Disaccharide Synthase (LpxB)-7 solubilizing mutations
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 68.016, 68.016, 155.139 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 55.070 - 2.100 |
R-factor | 0.1973 |
Rwork | 0.195 |
R-free | 0.22260 |
Structure solution method | SAD |
Data reduction software | XDS (June 17, 2015) |
Data scaling software | Aimless (0.1.27) |
Phasing software | PHENIX (v1.10_2155) |
Refinement software | PHENIX (v1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 155.140 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.066 | 0.997 |
Rpim | 0.041 | 0.615 |
Number of reflections | 24865 | 2012 |
<I/σ(I)> | 13.5 | 1.6 |
Completeness [%] | 99.4 | 99.9 |
Redundancy | 3.5 | 3.6 |
CC(1/2) | 0.998 | 0.550 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 292 | 39% PEG 4000, 0.1 M Tris-HCl pH 8.6, 0.7 M LiCl mixed 1:1 with 8 g/L protein in 0.3 M NaCl, 5% glycerol, 20 mM Tris-HCl pH 7.4, 5 mM DTT with 100 mM trimethylammonium chloride additive used at 10 mM or 10% of drop volume |