5W87
Crystal structure of the C-terminal lobe of the human HERC6 HECT domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298.15 |
| Detector technology | CCD |
| Collection date | 2017-03-27 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 |
| Unit cell lengths | 74.579, 74.579, 114.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.879 - 2.199 |
| R-factor | 0.2129 |
| Rwork | 0.211 |
| R-free | 0.24350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c4z |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.528 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: 000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.880 | 2.278 |
| High resolution limit [Å] | 2.199 | 2.199 |
| Rmerge | 0.057 | 0.758 |
| Rpim | 0.035 | 0.623 |
| Number of reflections | 11479 | 782 |
| <I/σ(I)> | 12.12 | |
| Completeness [%] | 91.8 | 64.41 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | K2HPO4/NaH2PO4, NaCl, imidazole:HCl |
