5W6V

The Structure of human Argonaute-1 in complex with the hook motif of human GW182

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	93.750, 136.861, 86.054
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.344 - 2.828
R-factor	0.2022
Rwork	0.200
R-free	0.24480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kre
RMSD bond length	0.002
RMSD bond angle	0.627
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	136.861	136.861	2.837
High resolution limit [Å]	2.828	13.121	2.828
Rmerge	0.079	0.023	0.549
Rmeas	0.092	0.028	0.634
Rpim	0.045	0.024	0.414
Number of reflections	27125
<I/σ(I)>	16.3		2.5
Completeness [%]	99.9		100
Redundancy	4		4.1
CC(1/2)	0.997	0.999	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	290.15	21.2% w/v PEG3350, 0.1 M di-ammonium tartrate