5W10
Lcd1 GAF domain in complex with cAMP ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-16 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9790 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 119.190, 119.995, 62.454 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.340 - 2.150 |
R-factor | 0.19617 |
Rwork | 0.195 |
R-free | 0.22519 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.547 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.078 | 0.560 |
Number of reflections | 93522 | |
<I/σ(I)> | 17.8 | 1.6 |
Completeness [%] | 99.6 | 99.2 |
Redundancy | 3.9 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M sodium citrate and 20% PEG 3350 |