5VZJ
STRUCTURE OF A TWELVE COMPONENT MPP6-NUCLEAR RNA EXOSOME COMPLEX BOUND TO RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-27 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 141.090, 213.592, 225.909 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.247 - 3.300 |
R-factor | 0.2192 |
Rwork | 0.217 |
R-free | 0.26590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4001 5k36 |
RMSD bond length | 0.001 |
RMSD bond angle | 0.398 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.247 | 150.000 | 3.420 |
High resolution limit [Å] | 3.300 | 7.110 | 3.300 |
Rmerge | 0.086 | 0.043 | 0.617 |
Rmeas | 0.101 | 0.049 | 0.754 |
Rpim | 0.051 | 0.024 | 0.424 |
Total number of observations | 343880 | ||
Number of reflections | 101546 | ||
<I/σ(I)> | 8.6 | ||
Completeness [%] | 97.3 | 98.8 | 94.7 |
Redundancy | 3.4 | 4.1 | 2.6 |
CC(1/2) | 0.996 | 0.142 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 11-13% PEG3350, 100mM NaCitrate pH 5.6, 7 mM MES pH 6.5, 175-200 mM ammonium sulfate. |