5VZJ
STRUCTURE OF A TWELVE COMPONENT MPP6-NUCLEAR RNA EXOSOME COMPLEX BOUND TO RNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Exosome complex component RRP45 | polymer | 305 | 34001.9 | 1 | UniProt (Q05636) Pfam (PF01138) Pfam (PF03725) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Ribosomal RNA-processing protein 45 |
| 2 | B (B) | Exosome complex component SKI6 | polymer | 250 | 28007.1 | 1 | UniProt (P46948) Pfam (PF01138) Pfam (PF03725) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Extracellular mutant protein 20,Ribosomal RNA-processing protein 41,Superkiller protein 6 |
| 3 | C (C) | Exosome complex component RRP43 | polymer | 394 | 44060.9 | 1 | UniProt (P25359) Pfam (PF01138) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Ribosomal RNA-processing protein 43 |
| 4 | D (D) | Exosome complex component RRP46 | polymer | 225 | 24574.3 | 1 | UniProt (P53256) Pfam (PF01138) Pfam (PF03725) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Ribosomal RNA-processing protein 46 |
| 5 | E (E) | Exosome complex component RRP42 | polymer | 269 | 29492.6 | 1 | UniProt (Q12277) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Ribosomal RNA-processing protein 42 |
| 6 | F (F) | Exosome complex component MTR3 | polymer | 250 | 27604.0 | 1 | UniProt (P48240) Pfam (PF01138) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | mRNA transport regulator 3 |
| 7 | G (G) | Exosome complex component RRP40 | polymer | 244 | 26990.8 | 1 | UniProt (Q08285) Pfam (PF21262) Pfam (PF15985) Pfam (PF18311) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Ribosomal RNA-processing protein 40 |
| 8 | H (H) | Exosome complex component RRP4 | polymer | 363 | 39877.6 | 1 | UniProt (P38792) Pfam (PF14382) Pfam (PF21266) Pfam (PF15985) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Ribosomal RNA-processing protein 4 |
| 9 | I (I) | Exosome complex component CSL4 | polymer | 296 | 32026.7 | 1 | UniProt (P53859) Pfam (PF21551) Pfam (PF10447) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | CEP1 synthetic lethal protein 4 |
| 10 | J (J) | Exosome complex exonuclease RRP6 | polymer | 559 | 64515.6 | 1 | UniProt (Q12149) Pfam (PF01612) Pfam (PF00570) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Ribosomal RNA-processing protein 6 |
| 11 | K (K) | Exosome complex exonuclease DIS3 | polymer | 1003 | 114056.0 | 1 | UniProt (Q08162) Pfam (PF13638) Pfam (PF17216) Pfam (PF17849) Pfam (PF00773) Pfam (PF17215) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Chromosome disjunction protein 3,Ribosomal RNA-processing protein 44 |
| 12 | L (L) | M-phase phosphoprotein 6 homolog | polymer | 42 | 4674.3 | 1 | UniProt (P53725) | Saccharomyces cerevisiae (Baker's yeast) | Exosome-associated RNA-binding protein MPP6 |
| 13 | M (M) | RNA (11-MER) | polymer | 11 | 3438.1 | 1 | Homo sapiens | ||
| 14 | N (N) | RNA (19-MER) | polymer | 19 | 5933.5 | 1 | Homo sapiens | ||
| 15 | O, Q, R, S, U (A, B, G, K) | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
| 16 | P, V (A, K) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 17 | T (K) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 18 | AA, BA, CA, DA, W... (G, H, J, K, A...) | water | water | 18.0 | 64 | Chemie (HOH) |
Sequence modifications
B: 1 - 246 (UniProt: P46948)
D: 1 - 223 (UniProt: P53256)
E: 1 - 265 (UniProt: Q12277)
G: 1 - 240 (UniProt: Q08285)
H: 1 - 359 (UniProt: P38792)
I: 1 - 292 (UniProt: P53859)
J: 129 - 684 (UniProt: Q12149)
K: 1 - 1001 (UniProt: Q08162)
L: 81 - 120 (UniProt: P53725)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Asp -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Pro -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Pro -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Pro -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Pro -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 126 | - | expression tag |
| Leu 127 | - | expression tag |
| Met 128 | - | expression tag |
| Asn 238 | Asp 238 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Asn 171 | Asp 171 | engineered mutation |
| Asn 551 | Asp 551 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 79 | - | expression tag |
| Ser 80 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 479253.3 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 729.9 | |
| All* | Total formula weight | 479983.2 |
