5VZ4
Receptor-growth factor crystal structure at 2.20 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 75.352, 88.768, 121.293 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.630 - 2.200 |
| R-factor | 0.1946 |
| Rwork | 0.191 |
| R-free | 0.25430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5VZ3 & 2GH0 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.682 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 71.630 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.085 | 0.045 | 0.577 |
| Rmeas | 0.094 | 0.051 | 0.644 |
| Rpim | 0.039 | 0.022 | 0.280 |
| Total number of observations | 118710 | ||
| Number of reflections | 20379 | ||
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 97.4 | 97.8 | 85.4 |
| Redundancy | 5.8 | 5.5 | 4.9 |
| CC(1/2) | 0.997 | 0.852 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 297 | 0.1 M Bis-Tris, pH 6.0, 1.5 M ammonium sulfate, 10% w/v ethylene glycol, 30-minute soak in 0.5 M sodium bromide |
