5VXZ

High-affinity AXL decoy receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	77.220, 80.470, 249.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.610 - 2.300
R-factor	0.2008
Rwork	0.198
R-free	0.24720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c5d
RMSD bond length	0.018
RMSD bond angle	1.995
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.610		2.360
High resolution limit [Å]	2.300	10.290	2.300
Rmerge	0.069	0.030	0.886
Number of reflections	69824
<I/σ(I)>	20.14	54.69	1.67
Completeness [%]	99.6	97.6	97.9
Redundancy	10.7		4.1
CC(1/2)	0.999	1.000	0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	295	0.7 M Li2SO4, 0.1 M Tris.HCl (pH 8.4)