5VND
Crystal structure of FGFR1-Y563C (FGFR4 surrogate) covalently bound to H3B-6527
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2015-11-16 |
Detector | RAYONIX |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 208.494, 58.925, 65.935 |
Unit cell angles | 90.00, 107.59, 90.00 |
Refinement procedure
Resolution | 99.370 - 2.200 |
R-factor | 0.18059 |
Rwork | 0.178 |
R-free | 0.22333 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v05 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.896 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.370 | 2.150 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 1.000 | |
Rpim | 0.532 | |
Total number of observations | 4591 | |
Number of reflections | 46172 | 4550 |
<I/σ(I)> | 8.6 | 3.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG10000, 0.1M MES pH6.2, 0.3M Ammonium Sulphate, 5% Ethylene glycol |