5VHE

DHX36 in complex with the c-Myc G-quadruplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	72.526, 79.282, 212.077
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.641 - 3.793
R-factor	0.2427
Rwork	0.238
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vha
RMSD bond length	0.002
RMSD bond angle	0.496
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	39.641
High resolution limit [Å]	3.793
Rmerge	0.275
Rpim	0.092
Number of reflections	12615
<I/σ(I)>	7.81
Completeness [%]	99.2
Redundancy	9.6
CC(1/2)	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294.15	200 mM sodium malonate (pH 7.0) and 25% w/v PEG3350