5VGB
Crystal structure of NmeCas9 HNH domain bound to anti-CRISPR AcrIIC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.115830 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.164, 60.487, 77.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.641 - 1.497 |
| R-factor | 0.1665 |
| Rwork | 0.164 |
| R-free | 0.20980 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.816 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.640 | 47.640 | 1.520 |
| High resolution limit [Å] | 1.490 | 8.180 | 1.490 |
| Rmerge | 0.103 | 0.028 | 2.505 |
| Rmeas | 0.113 | 0.031 | 2.764 |
| Rpim | 0.044 | 0.012 | 1.141 |
| Total number of observations | 228832 | ||
| Number of reflections | 35654 | 277 | 1526 |
| <I/σ(I)> | 9.8 | 29.5 | 1 |
| Completeness [%] | 99.4 | ||
| Redundancy | 6.4 | 5.9 | 5.5 |
| CC(1/2) | 0.998 | 0.999 | 0.411 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.25 | 291.15 | 200 mM Ammonium sulfate, 0.1 M Sodium acetate, 22% PEG 4,000 |
