5VG9
Structure of the eukaryotic intramembrane Ras methyltransferase ICMT (isoprenylcysteine carboxyl methyltransferase) without a monobody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2015-04-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.640, 123.650, 236.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.198 - 4.000 |
R-factor | 0.3705 |
Rwork | 0.370 |
R-free | 0.38860 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.453 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.198 | 4.070 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.071 | |
Rpim | 0.012 | 0.341 |
Number of reflections | 6119 | 309 |
<I/σ(I)> | 85 | 3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 34.6 | 35.6 |
CC(1/2) | 0.787 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 26% PEG400 200 mM CaCl2 50 mM Na acetate, pH 5.5 |