5VEO
Murine ectonucleotide pyrophosphatase / phosphodiesterase 5 (ENPP5, NPP5), inactive (T72A), in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9768 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 84.239, 84.239, 295.796 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.980 - 1.530 |
| R-factor | 0.1153 |
| Rwork | 0.115 |
| R-free | 0.14490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.157 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.530 |
| Number of reflections | 93929 |
| <I/σ(I)> | 37.3 |
| Completeness [%] | 99.0 |
| Redundancy | 15.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | sodium acetate, PEG 200, NAD+ |
