5VEM
Human ectonucleotide pyrophosphatase / phosphodiesterase 5 (ENPP5, NPP5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-17 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9801 |
Spacegroup name | P 31 |
Unit cell lengths | 109.411, 109.411, 134.820 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.940 - 2.600 |
R-factor | 0.1973 |
Rwork | 0.196 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.667 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.600 |
Number of reflections | 55639 |
<I/σ(I)> | 10.5 |
Completeness [%] | 100.0 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | MES, PEG 6000, MPD |