5VDP
Human cyclic GMP-AMP synthase (cGAS) in complex with 2',3'-cGAMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 173 |
Detector technology | PIXEL |
Collection date | 2016-07-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 215.886, 46.733, 88.165 |
Unit cell angles | 90.00, 110.45, 90.00 |
Refinement procedure
Resolution | 32.446 - 2.300 |
R-factor | 0.1995 |
Rwork | 0.197 |
R-free | 0.24290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o67 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.004 |
Data reduction software | Aimless |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | PHENIX (dev_1999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.446 | |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 37195 | |
<I/σ(I)> | 9.3 | |
Completeness [%] | 100.0 | |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 18-20% PEG 3350, 0.2 M ammonium citrate pH 7 |