5VAM
BRAF in Complex with RAF709
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 109.390, 109.390, 135.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.750 - 2.100 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.6) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.920 | 48.920 | 2.160 |
High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
Rmerge | 0.200 | 0.028 | 1.710 |
Rmeas | 0.216 | 0.030 | 1.871 |
Rpim | 0.081 | 0.012 | 0.754 |
Total number of observations | 322160 | 4688 | 23899 |
Number of reflections | 45681 | ||
<I/σ(I)> | 10.1 | 45.8 | 1.1 |
Completeness [%] | 93.6 | 99.5 | 100 |
Redundancy | 7.1 | 6.2 | 6.1 |
CC(1/2) | 0.995 | 1.000 | 0.402 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 291 | 100 mM Tris ph 8.4, 12% PEG8000, and 50mM NaCl |