5VAD
Crystal structure of human Prolyl-tRNA synthetase (PRS) in complex with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.089, 92.278, 84.832 |
| Unit cell angles | 90.00, 111.18, 90.00 |
Refinement procedure
| Resolution | 19.930 - 2.360 |
| R-factor | 0.1991 |
| Rwork | 0.196 |
| R-free | 0.26160 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.549 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.410 |
| High resolution limit [Å] | 2.360 | 6.430 | 2.370 |
| Rmerge | 0.071 | 0.047 | 0.660 |
| Number of reflections | 41903 | ||
| <I/σ(I)> | 11.4 | ||
| Completeness [%] | 99.8 | 99.5 | 97 |
| Redundancy | 3.7 | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% PEG3350, 500mM CaCl2, Hepes pH7.5 |
