5V7P
Atomic structure of the eukaryotic intramembrane Ras methyltransferase ICMT (isoprenylcysteine carboxyl methyltransferase), in complex with a monobody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2015-11-09 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9790 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.554, 87.691, 147.681 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.772 - 2.300 |
R-factor | 0.2156 |
Rwork | 0.214 |
R-free | 0.24720 |
Structure solution method | SIRAS |
RMSD bond length | 0.003 |
RMSD bond angle | 0.595 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.000 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.200 | 0.979 |
Rpim | 0.062 | 0.295 |
Number of reflections | 23204 | 1197 |
<I/σ(I)> | 13.3 | 2.5 |
Completeness [%] | 97.2 | 99.8 |
Redundancy | 11.2 | 11.1 |
CC(1/2) | 0.863 | 0.547 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 40/60% (v/v ICMT:9.9 monoacylgylcerol) 30% PEG 400 100 mM NaCl 100 mM Na HEPES, pH 7.5 |