5V7P

Atomic structure of the eukaryotic intramembrane Ras methyltransferase ICMT (isoprenylcysteine carboxyl methyltransferase), in complex with a monobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	90
Detector technology	PIXEL
Collection date	2015-11-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9790
Spacegroup name	P 21 21 21
Unit cell lengths	40.554, 87.691, 147.681
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.772 - 2.300
R-factor	0.2156
Rwork	0.214
R-free	0.24720
Structure solution method	SIRAS
RMSD bond length	0.003
RMSD bond angle	0.595
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.000	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.200	0.979
Rpim	0.062	0.295
Number of reflections	23204	1197
<I/σ(I)>	13.3	2.5
Completeness [%]	97.2	99.8
Redundancy	11.2	11.1
CC(1/2)	0.863	0.547

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	40/60% (v/v ICMT:9.9 monoacylgylcerol) 30% PEG 400 100 mM NaCl 100 mM Na HEPES, pH 7.5