5V4W
Human glucokinase in complex with novel indazole activator.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.594 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.709, 78.873, 120.333 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.940 - 2.390 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.26100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.940 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.458 |
| Number of reflections | 19113 | |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 99.1 | 89.6 |
| Redundancy | 5.8 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 15% PEG3350, 200 mM ammonium iodide, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
