5UY3
Crystal structure of human Fab PGT144, a broadly reactive and potent HIV-1 neutralizing antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 |
Unit cell lengths | 57.600, 67.550, 69.550 |
Unit cell angles | 82.04, 74.89, 81.26 |
Refinement procedure
Resolution | 36.293 - 2.900 |
R-factor | 0.243 |
Rwork | 0.241 |
R-free | 0.28210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u1s |
RMSD bond length | 0.003 |
RMSD bond angle | 0.619 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.160 | 0.390 |
Number of reflections | 21292 | |
<I/σ(I)> | 5.5 | 1.9 |
Completeness [%] | 96.6 | 96.9 |
Redundancy | 2.1 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | JCSG F8 |