5UXH
Structure of Human POFUT1 in complex with GDP-fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.230, 133.920, 70.010 |
| Unit cell angles | 90.00, 104.13, 90.00 |
Refinement procedure
| Resolution | 48.709 - 2.410 |
| R-factor | 0.243 |
| Rwork | 0.240 |
| R-free | 0.29240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ux6 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.865 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Number of reflections | 33916 | 3207 |
| <I/σ(I)> | 5.28 | |
| Completeness [%] | 98.0 | 92.1 |
| Redundancy | 4.5 | 4 |
| CC(1/2) | 0.988 | 0.441 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 16.5% v/v PEG3350, 0.05% w/v octyl-beta-D-glucoside, 0.5% v/v glycerol, and 100 mM sodium citrate pH 5.0. The ligand-bound structure was obtained by soaking crystals for 15 minutes in cryopreservation solution containing 2 mM GDP-Fucose immediately prior to freezing |
