5UXC
Crystal structure of macrolide 2'-phosphotransferase MphH from Brachybacterium faecium in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.556, 77.002, 94.409 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.244 - 1.720 |
| R-factor | 0.1661 |
| Rwork | 0.164 |
| R-free | 0.20740 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.497 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2733: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.000 | 1.820 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rpim | 0.056 | 0.345 |
| Number of reflections | 39085 | 6227 |
| <I/σ(I)> | 9.1 | 2.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4 | 4.1 |
| CC(1/2) | 0.994 | 0.805 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 2 M ammonium sulfate, 2% PEG 400, 0.1 M HEPES pH 7.5, 1% trehalose, 5 mM azithromycin, 5 mM GMP-PNP |
