5UT0
JAK2 JH2 in complex with AT9283
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-14 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.135, 57.681, 60.443 |
Unit cell angles | 90.00, 109.95, 90.00 |
Refinement procedure
Resolution | 41.486 - 2.102 |
R-factor | 0.1706 |
Rwork | 0.168 |
R-free | 0.22580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fvp |
RMSD bond length | 0.003 |
RMSD bond angle | 0.604 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.486 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 16717 | |
<I/σ(I)> | 8.9 | 1.9 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 3.6 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1M Tris pH 8.0 0.2M Sodium acetate 12-20% PEG 4,000 |