5USW
The crystal structure of 7,8-dihydropteroate synthase from Vibrio fischeri ES114
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-08 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.953, 65.881, 94.942 |
Unit cell angles | 90.00, 105.83, 90.00 |
Refinement procedure
Resolution | 36.130 - 1.643 |
R-factor | 0.1729 |
Rwork | 0.171 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jq9 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.268 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.670 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmerge | 0.055 | 0.831 |
Rpim | 0.037 | 0.592 |
Number of reflections | 130804 | 6479 |
<I/σ(I)> | 13.8 | 1.5 |
Completeness [%] | 98.5 | 97.7 |
Redundancy | 3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M potassium sodium tartrate, 20% w/v PEG3350 |