5URV

Crystal structure of Frizzled 7 CRD in complex with C24 fatty acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	93
Detector technology	PIXEL
Collection date	2015-01-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 2
Unit cell lengths	97.560, 104.902, 41.351
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.351 - 2.200
R-factor	0.1653
Rwork	0.163
R-free	0.21030
RMSD bond length	0.011
RMSD bond angle	1.325
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	22293
<I/σ(I)>	14.4
Completeness [%]	99.9
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.1M MES sodium salt pH 6.5, 2.0M Ammonium sulfate, 5%(w/v) PEG 400