5UN6
Frizzled-8 complex with designed surrogate Wnt agonist, A1 dataset
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 116.420, 36.450, 125.530 |
Unit cell angles | 90.00, 93.73, 90.00 |
Refinement procedure
Resolution | 19.816 - 3.200 |
R-factor | 0.2047 |
Rwork | 0.200 |
R-free | 0.24750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f0a calculated model of B12 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.632 |
Data reduction software | XDS (March 30, 2013) |
Data scaling software | XSCALE (June 17, 2015) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.760 | 3.418 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 17882 | 800 |
<I/σ(I)> | 12.88 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 4.1 | |
CC(1/2) | 0.996 | 0.829 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 20% PEG 3000, 0.1 M Sodium citrate pH 5.5 |