5UN2
Crystal Structure of Mouse Cadherin-23 EC19-21 with non-syndromic deafness (DFNB12) associated mutation R2029W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-18 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.542 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 189.475, 73.908, 49.375 |
| Unit cell angles | 90.00, 96.62, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.960 |
| R-factor | 0.19818 |
| Rwork | 0.196 |
| R-free | 0.23582 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tfk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.440 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.010 |
| High resolution limit [Å] | 2.960 | 2.960 |
| Rmerge | 0.164 | 0.554 |
| Rmeas | 0.187 | 0.631 |
| Rpim | 0.087 | 0.294 |
| Number of reflections | 14294 | 667 |
| <I/σ(I)> | 7.863 | 1.97 |
| Completeness [%] | 91.5 | 95.3 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277 | 0.1 M HEPES pH 7.3 0.1 M CaCl2 27% PEG 400 5% glycerol |
