5UN1

Crystal structure of GluN1/GluN2B delta-ATD NMDA receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-22
Detector	ADSC HF-4M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	181.570, 108.470, 182.460
Unit cell angles	90.00, 111.44, 90.00

Refinement procedure

Resolution	49.994 - 3.600
R-factor	0.2892
Rwork	0.288
R-free	0.31620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tll
RMSD bond length	0.006
RMSD bond angle	0.818
Data reduction software	XDS (Nov 1, 2016)
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX ((dev_2597: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.680
High resolution limit [Å]	3.580	3.580
Rmerge	0.085	0.570
Number of reflections	76987	2414
<I/σ(I)>	8.33
Completeness [%]	86.0	38.1
Redundancy	5.3	4.08
CC(1/2)	0.990	0.120

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	2-ethanesulfonic acid, sodium fluoride, PEG 3350