5ULG
Crystal structure of RPE65 in complex with MB-008 and palmitate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 65 |
| Unit cell lengths | 175.319, 175.319, 86.653 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.850 - 2.100 |
| R-factor | 0.17719 |
| Rwork | 0.176 |
| R-free | 0.20722 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4rsc |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.541 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.152 |
| Number of reflections | 86411 |
| <I/σ(I)> | 9.34 |
| Completeness [%] | 98.0 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 281 | 40% PEG 300, 200 mM NaCl, 100 mM CHES, pH 9.5 |
