5UKL

Human GRK2 in complex with Gbetagamma subunits and CCG222886 (14bd)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-28
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	C 1 2 1
Unit cell lengths	194.255, 71.429, 111.259
Unit cell angles	90.00, 110.51, 90.00

Refinement procedure

Resolution	30.000 - 2.150
R-factor	0.1844
Rwork	0.182
R-free	0.22750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v5w
RMSD bond length	0.018
RMSD bond angle	1.902
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.190
High resolution limit [Å]	2.150	5.830	2.150
Rmerge	0.161	0.078
Number of reflections	77337
<I/σ(I)>	24.9
Completeness [%]	100.0	99.8	100
Redundancy	18.3	18.8	11.2
CC(1/2)		0.997	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	50 mM MES, 0.8-1.2 M sodium chloride, 8-16% PEG3350