5UKK
Human GRK2 in complex with human G-beta-gamma subunits and CCG211998 (14ak)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97849 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 113.123, 62.423, 102.035 |
| Unit cell angles | 90.00, 92.81, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.2237 |
| Rwork | 0.221 |
| R-free | 0.28070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v5w |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.531 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
| Rmerge | 0.098 | 0.038 | 0.849 |
| Number of reflections | 39963 | ||
| <I/σ(I)> | 9.24 | 0.83 | |
| Completeness [%] | 92.7 | 85.7 | 94.3 |
| Redundancy | 2.6 | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 50 mM MES, 8-16% PEG3350, 0.8-1.2 M sodium chloride |
