5UKK

Human GRK2 in complex with human G-beta-gamma subunits and CCG211998 (14ak)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97849
Spacegroup name	P 1 2 1
Unit cell lengths	113.123, 62.423, 102.035
Unit cell angles	90.00, 92.81, 90.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.2237
Rwork	0.221
R-free	0.28070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v5w
RMSD bond length	0.011
RMSD bond angle	1.531
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.098	0.038	0.849
Number of reflections	39963
<I/σ(I)>	9.24		0.83
Completeness [%]	92.7	85.7	94.3
Redundancy	2.6	2.6	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	50 mM MES, 8-16% PEG3350, 0.8-1.2 M sodium chloride