5UJJ
Crystal structure of human H130R tryptophanyl-tRNA synthetase in complex with TrpAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.352, 95.277, 99.623 |
| Unit cell angles | 90.00, 130.46, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.2163 |
| Rwork | 0.215 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qui |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.113 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.800 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.057 | 0.170 |
| Number of reflections | 56074 | 2837 |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 99.2 | 99.6 |
| Redundancy | 6.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 18% PEG1500, 0.1mol/L Bis-Tris pH6.5 |
