5UJI
Crystal structure of human T2-Tryptophanyl-tRNA synthetase with H130R mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9870 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.888, 139.057, 146.724 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.726 - 2.790 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.26430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qui |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.158 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 146.000 | 2.850 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.093 | 0.471 |
| Number of reflections | 21588 | 1066 |
| <I/σ(I)> | 6.5 | |
| Completeness [%] | 98.9 | 99.4 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 20 % PEG4000, 0.1 M Na Citrate pH5.0, 0.1 M MgCl2 |
