5UIV
Structure of Thymidylate Kinase from Candida albicans Reveals Origin of Broad Substrate Specificity and a Novel Structural Element.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-19 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 69.965, 69.965, 116.161 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.590 - 2.450 |
| R-factor | 0.17049 |
| Rwork | 0.168 |
| R-free | 0.21805 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tmk |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.938 |
| Data reduction software | iMOSFLM (7.2.0) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.590 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.134 | 0.278 |
| Number of reflections | 11717 | |
| <I/σ(I)> | 1.89 | |
| Completeness [%] | 92.1 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 285 | 0.1 M HEPES, 1.1 N Sodium Citrate, pH 7.5 |
