5UDP
High resolution x-ray crystal structure of synthetic insulin lispro
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-11 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.644, 61.470, 59.148 |
Unit cell angles | 90.00, 110.60, 90.00 |
Refinement procedure
Resolution | 25.718 - 1.348 |
R-factor | 0.1613 |
Rwork | 0.160 |
R-free | 0.19430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zs2 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.765 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (dev_2283) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.370 | |
High resolution limit [Å] | 1.348 | 3.660 | 1.348 |
Rmerge | 0.068 | 0.073 | 0.777 |
Number of reflections | 67860 | ||
<I/σ(I)> | 6.5 | ||
Completeness [%] | 98.3 | 98.1 | 90 |
Redundancy | 6.2 | 6.6 | 3.5 |
CC(1/2) | 0.993 | 0.772 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 292 | 0.3 M TRIS, 0.5 M sodium sulfate, 0.6 mM Zinc acetate, 0.06% phenol |