5UCJ
Hsp90b N-terminal domain with inhibitors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-27 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 65 |
| Unit cell lengths | 129.464, 129.464, 106.357 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.377 - 1.693 |
| R-factor | 0.1749 |
| Rwork | 0.174 |
| R-free | 0.19660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uym |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.004 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.690 | 1.700 |
| Number of reflections | 112035 | |
| <I/σ(I)> | 23 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 30% PEG 8,000, 0.2 M sodium acetate, 0.1 sodium cacodylate pH 6.5 |
