5U9G
3.2 A cryo-EM ArfA-RF2 ribosome rescue complex (Structure I)
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | DIRECT ELECTRON DE-20 (5k x 3k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.2 |
| Number of Particle | 539311 |
| Software | EMAN2,PHENIX,FREALIGN,Leginon,CTFFIND4,UCSF Chimera,RSRef |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
