5U8C
CRYSTAL STRUCTURE OF GLUN1/GLUN2A LIGAND-BINDING DOMAIN IN COMPLEX WITH GLYCINE AND NVP-AAM077
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.550, 84.227, 121.218 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.196 - 1.598 |
R-factor | 0.1893 |
Rwork | 0.189 |
R-free | 0.20410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nf8 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.930 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.598 |
Rmerge | 0.077 |
Number of reflections | 72757 |
<I/σ(I)> | 17.84 |
Completeness [%] | 89.5 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 18% Polyethylene glycol monoethylether 2000 (PEG2000 MME), 100mM HEPES-NaOH pH 7.0, and 75mM NaCl |